Here we illustrate the use of DIFFRAC.EVA to give a measure of grain size, shape and colour by analysis of wheat and barley grains. The software provides a measure of grain size, and gives a measure of variation within a sample. From this, it is possible to perform a full analysis of variance of the data. A sample of barley grain was measured, and the software produced a measure of the colour component as well as measures of grain size and shape.
DIFFRAC.EVA (diffraction enhanced X-ray fluorescence) is a simple and fast way of performing a detailed measure of grain size, shape and colour that is fast enough to be incorporated into a breeding program. Using DIFFRAC.EVA, similar grain size and shape characteristics as those measured using the SKCS can be obtained at a reduced cost and with a much higher throughput. The SKCS can be used as a reference for comparison, providing an accuracy check for the data obtained using DIFFRAC.EVA.
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