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Bacteriology Laboratory Protocols is an excellent handbook for the microbiology laboratory. This book should not only prove useful to the new laboratory scientist but also to the microbiology laboratory staff with an interest in carrying out the outlined procedures. The book serves as a comprehensive reference for the student or the laboratory staff who is interested in carrying out microbiological procedures in the laboratory.
Mechanistic and structural aspects of the serotonin transporter are elucidated using a proteomics and molecular biology approach.
Hereditary high-affinity human dopamine transporter (DAT) variants alter the pharmacology of psychomotor stimulants and psychostimulant-like drugs. To elucidate the mechanism of action of the DAT, we have identified proteolytic fragments of the membrane-bound human serotonin transporter (hSERT) and assessed their functional properties. These proteolytic fragments are generated in purified transporter, a cell-free system, or in live cells by the action of membrane-bound endoproteases. We have used neuroblastoma cells as a model cell line that endogenously expresses hSERT. Endoproteolytic fragments of hSERT purified from cells are more stable than those extracted from a cell-free system. In the cell-free system, a protease cleaves hSERT at two discrete sites: one at the amino terminus of the transporter and the other at the central region of the protein. The cleavage of the amino terminus, but not the central region, results in the loss of high-affinity serotonin uptake activity.
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